CD99 anticorps

N° du produit ABIN7652341

Cet anticorps Souris Monoclonal détecte spécifiquement CD99 dans IHC et IHC (fp). Il présente une réactivité envers Humain.

Aperçu rapide pour CD99 anticorps (ABIN7652341)

Antigène: CD99

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 12E7 MIC2-877

Détail du produit anti-CD99 anticorps

Fonction: CD99 / MIC2 (Ewing's Sarcoma Marker)(12E7 + MIC2/877)

Attributs du produit: This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogène: Human acute lymphocytic leukemia T-cells (12E7), Recombinant human MIC2 protein (MIC2/877)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Flow cytometry: 5-10 uL/million cells. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 1:100-1:200 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Immunofluorescence 1:100-1:200. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MOLT-4 cells. Pancreas or Ewing sarcoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet:

Synonyms: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

Gene Symbol: CD99

Tissue Expression: Lymphocytes|Ovary|Pancreatic islet cells|T-cells|Testis

Poids moléculaire: 27-32 kDa

ID gène: 4267

UniProt: P14209