EPX anticorps (CF®740)

N° du produit ABIN7653523

Cet anticorps Souris Monoclonal détecte spécifiquement EPX dans IF. Il présente une réactivité envers Humain.

Aperçu rapide pour EPX anticorps (CF®740) (ABIN7653523)

Antigène: EPX (Eosinophil Peroxidase (EPX))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPX est conjugé à/à la CF®740

Application: Immunofluorescence (IF)

Clone: AHE-1

Détail du produit anti-EPX anticorps (CF®740)

Fonction: Eosinophil Peroxidase(AHE-1), CF740 conjugate

Attributs du produit: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human eosinophils from a patient with hypereosinophilic syndrome

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7 cells. Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur EPX

Antigène: EPX (Eosinophil Peroxidase (EPX))

Autre désignation: Eosinophil Peroxidase

Sujet:

Synonyms: Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE

Gene Symbol: EPX

Tissue Expression: Eosinophils

Poids moléculaire: 70 kDa (55 kDa-Heavy Chain, 15 kDa-Light Chain)

ID gène: 8288

UniProt: P11678