ESR2 anticorps (C-Term) (CF®740)

N° du produit ABIN7653848

L’anticorps Souris Monoclonal anti-ESR2 a été validé pour WB, IHC, FACS et IHC (fp). Il convient pour détecter ESR2 dans des échantillons de Humain, Souris, Rat, Mouton, Porc, Cheval et Singe.

Aperçu rapide pour ESR2 anticorps (C-Term) (CF®740) (ABIN7653848)

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain, Souris, Rat, Mouton, Porc, Cheval, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ESR2 est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ERb455

Détail du produit anti-ESR2 anticorps (CF®740)

Épitope: C-Term

Fonction: Estrogen Receptor beta 1(ERb455), CF740 conjugate

Attributs du produit: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: C-terminus fragment of recombinant human estrogen receptor beta protein

Isotype: IgG2a

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur ESR2

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: Estrogen Receptor Beta 1

Sujet:

Synonyms: Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4

Gene Symbol: ESR2

Poids moléculaire: 53-59 kDa

ID gène: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling