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Fibronectin anticorps (CF®640R)

Cet anticorps anti-Fibronectin est un anticorps Souris Monoclonal détectant Fibronectin dans IHC et IHC (fp). Adapté pour Humain.
N° du produit ABIN7654038

Aperçu rapide pour Fibronectin anticorps (CF®640R) (ABIN7654038)

Antigène

Voir toutes Fibronectin Anticorps
Fibronectin

Reactivité

  • 141
  • 58
  • 56
  • 26
  • 15
  • 9
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
Humain

Hôte

  • 91
  • 86
  • 5
  • 2
Souris

Clonalité

  • 101
  • 81
  • 1
Monoclonal

Conjugué

  • 77
  • 27
  • 10
  • 7
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Fibronectin est conjugé à/à la CF®640R

Application

  • 99
  • 63
  • 43
  • 42
  • 31
  • 26
  • 23
  • 23
  • 19
  • 19
  • 17
  • 13
  • 10
  • 10
  • 7
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  • 3
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone

568
  • Fonction

    Fibronectin (8th Type III Repeat)(568), CF640R conjugate

    Attributs du produit

    The epitope of this antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

    Immunogène

    High molecular weight proteins secreted by cultivated human fibroblasts

    Isotype

    IgG1, kappa
  • Indications d'application

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    Commentaires

    Positive Control: SW156 cells. Kidney.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.1 % BSA, 0.05 % azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect from light

    Stock

    4 °C

    Stockage commentaire

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    Date de péremption

    24 months
  • Antigène

    Fibronectin

    Sujet

    Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

    Gene Symbol: FN1

    Poids moléculaire

    220 kDa (monomer), 440 kDa (dimer)

    ID gène

    2335

    UniProt

    P02751
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