Fibronectin anticorps (CF®640R)
Aperçu rapide pour Fibronectin anticorps (CF®640R) (ABIN7654038)
Antigène
Voir toutes Fibronectin AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- Fibronectin (8th Type III Repeat)(568), CF640R conjugate
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Attributs du produit
- The epitope of this antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.
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Immunogène
- High molecular weight proteins secreted by cultivated human fibroblasts
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Isotype
- IgG1, kappa
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Indications d'application
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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Commentaires
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Positive Control: SW156 cells. Kidney.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1 mg/mL
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Buffer
- PBS, 0.1 % BSA, 0.05 % azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Protect from light
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Stock
- 4 °C
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Stockage commentaire
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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Date de péremption
- 24 months
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- Fibronectin
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Sujet
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Synonyms: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3
Gene Symbol: FN1
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Poids moléculaire
- 220 kDa (monomer), 440 kDa (dimer)
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ID gène
- 2335
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UniProt
- P02751
Antigène
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