Recombinant GFAP anticorps (CF®594)

N° du produit ABIN7654390

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, IHC, FACS et IHC (fp). Il présente une réactivité avec des échantillons de Humain, Rat, Souris, Boeuf (Vache), Porc, Lapin et Poulet.

Aperçu rapide pour Recombinant GFAP anticorps (CF®594) (ABIN7654390)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Porc, Lapin, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est conjugé à/à la CF®594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RASTRO-789

Détail du produit anti-GFAP anticorps (CF®594)

Fonction: GFAP (Astrocyte & Neural Stem Cell Marker) (rASTRO/789), CF594 conjugate

Attributs du produit: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human GFAP protein

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Brain or Astrocytoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet:

Synonyms: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Gene Symbol: GFAP

Tissue Expression: Glia

Poids moléculaire: ~50 kDa

ID gène: 2670

UniProt: P14136