Recombinant GP2 anticorps (AA 35-179) (Biotin)

N° du produit ABIN7654572

Cet anticorps anti-GP2 Monoclonal Lapin conjugué à Biotin (Clone GP2-2569R) (ABIN7654572) détecte spécifiquement GP2 dans WB, IHC et IHC (fp). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant GP2 anticorps (AA 35-179) (Biotin) (ABIN7654572)

Antigène: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp GP2 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GP2-2569R

Détail du produit anti-GP2 anticorps (Biotin)

Épitope: AA 35-179

Fonction: GP2 (Glycoprotein 2) / ZAP75 (GP2/2569R), Biotin conjugate

Attributs du produit: GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human GP2 protein fragment (around aa35-179)

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with mouse. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: PANC-1 cells. Pancreas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur GP2

Antigène: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Autre désignation: GP2

Classe de substances: Viral Protein

Sujet:

Synonyms: Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75

Gene Symbol: GP2

Tissue Expression: M cells

Poids moléculaire: 59 kDa

ID gène: 2813

UniProt: P55259