Recombinant HCG beta anticorps (CF®647)

N° du produit ABIN7654766

L’anticorps Souris Monoclonal anti-HCG beta a été validé pour IHC et IHC (fp). Il convient pour détecter HCG beta dans des échantillons de Humain.

Aperçu rapide pour Recombinant HCG beta anticorps (CF®647) (ABIN7654766)

Antigène: HCG beta

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HCG beta est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RHCGb-54

Détail du produit anti-HCG beta anticorps (CF®647)

Fonction: HCG-beta (Pregnancy & Choriocarcinoma Marker) (rHCGb/54), CF647 conjugate

Attributs du produit: This MAb reacts with a protein of 22 kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the i± and beta subunits. The i± subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human hCG beta protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: JAR or TT Cells. Placenta

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur HCG beta

Antigène: HCG beta

Sujet:

Synonyms: CG-beta, CGB3, CGB5, CGB7, CGB8, Choriogonadotropin Subunit beta, hCGB

Gene Symbol: CGB3

Tissue Expression: Placenta

Poids moléculaire: 22 kDa

ID gène: 1082

UniProt: P01233