HSPG anticorps (CF®740)

N° du produit ABIN7654934

L’anticorps Rat Monoclonal anti-HSPG a été validé pour IHC et IHC (fp). Il convient pour détecter HSPG dans des échantillons de Humain, Boeuf (Vache), Souris, Poisson, Singe et Porc.

Aperçu rapide pour HSPG anticorps (CF®740) (ABIN7654934)

Antigène: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reactivité: Humain, Boeuf (Vache), Souris, Poisson, Singe, Porc

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPG est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: A7L6

Détail du produit anti-HSPG anticorps (CF®740)

Fonction: Heparan Sulphate Proteoglycan(A7L6), CF740 conjugate

Attributs du produit: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Murine EHS laminin preparation

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Breast carcinomas, squamous cell carcinomas.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur HSPG

Antigène: HSPG (Heparan Sulphate Protoglycans (HSPG))

Autre désignation: Heparan Sulfate Proteoglycan

Sujet:

Synonyms: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1

Gene Symbol: HSPG2

Poids moléculaire: >400 kDa

ID gène: 3339

UniProt: P98160