Involucrin anticorps (CF®740)

N° du produit ABIN7655751

Cet anticorps anti-Involucrin est un anticorps Souris Monoclonal détectant Involucrin dans IHC et IHC (fp). Adapté pour Humain, Chien, Porc, Gorilla, Singe et Owl.

Aperçu rapide pour Involucrin anticorps (CF®740) (ABIN7655751)

Antigène: Involucrin (IVL)

Reactivité: Humain, Chien, Porc, Gorilla, Singe, Owl

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SY5

Détail du produit anti-Involucrin anticorps (CF®740)

Fonction: Involucrin(SY5), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified involucrin from human keratinocytes

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.1-0.2 μg/mL. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence. Does not react with mouse, others not known. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet:

Synonyms: IVL

Gene Symbol: IVL

Poids moléculaire: 66-170 kDa

ID gène: 3713

UniProt: P07476