KIP2 anticorps (CF®640R)

N° du produit ABIN7656298

Cet anticorps Souris Monoclonal détecte spécifiquement KIP2 dans IHC et IHC (fp). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour KIP2 anticorps (CF®640R) (ABIN7656298)

Antigène: KIP2

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 57P06

Détail du produit

Fonction: p57Kip2 (Mitotic Inhibitor/Suppressor Protein)(57P06), CF640R conjugate

Attributs du produit: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Immunogène: Recombinant full-length human p57Kip2 protein

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.25-0.5 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détail du antigène

Antigène: KIP2

Sujet:

Synonyms: Beckwith Wiedemann syndrome (WBS), BWCR, Cyclin dependent kinase inhibitor 1C (CDKN1C), KIP2, p57

Gene Symbol: CDKN1C

Poids moléculaire: 57 kDa

ID gène: 1028

UniProt: P49918