LAG3 anticorps (CF®488A)

N° du produit ABIN7656421

Cet anticorps Souris Monoclonal détecte spécifiquement LAG3 dans . Il présente une réactivité envers Humain.

Aperçu rapide pour LAG3 anticorps (CF®488A) (ABIN7656421)

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAG3 est conjugé à/à la CF®488A

Application: Veuillez nous consulter SVP

Clone: LAG3-3261

Détail du produit anti-LAG3 anticorps (CF®488A)

Fonction: Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)(LAG3/3261), CF488A conjugate

Attributs du produit: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4 CD8 T cells and NK cell, with shared homology in structure to CD4 Molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3 CD4 CD8 T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3 cells) and inhibit induced (i. e. antigen-specific) Treg suppressive activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human LAG3 protein

Isotype: IgG2a, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: Human tonsil or Hodgkin lymphoma (IHC-P).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur LAG3

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Autre désignation: Lymphocyte Activation Gene 3

Sujet:

Synonyms: CD223, FDC protein, LAG-3

Gene Symbol: LAG3

Tissue Expression: NK cells|T-cells

Poids moléculaire: 70 kDa

ID gène: 3902

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints