MHC Class II anticorps (CF®740)

N° du produit ABIN7656450

Cet anticorps anti-MHC Class II est un anticorps Souris Monoclonal détectant MHC Class II dans FACS, IP, Func et ELISA. Adapté pour Humain.

Aperçu rapide pour MHC Class II anticorps (CF®740) (ABIN7656450)

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), ELISA

Clone: BRA-FB6

Détail du produit anti-MHC Class II anticorps (CF®740)

Fonction: MHC II (HLA-DP) (beta chain)(BRA-FB6), CF740 conjugate

Attributs du produit: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MHC Class II

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Autre désignation: MHC-II

Sujet:

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Gene Symbol: HLA-DPB1

Poids moléculaire: 33-35 kDa

ID gène: 3115

UniProt: P04440