MHC Class II anticorps (CF®740)

N° du produit ABIN7656451

Cet anticorps Souris Monoclonal détecte spécifiquement MHC Class II dans FACS, WB, IP, Func et IF. Il présente une réactivité envers Humain et Porc.

Aperçu rapide pour MHC Class II anticorps (CF®740) (ABIN7656451)

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunofluorescence (IF)

Clone: SPV-L3

Détail du produit anti-MHC Class II anticorps (CF®740)

Fonction: MHC II (HLA-DQ)(SPV-L3), CF740 conjugate

Attributs du produit: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: T4-positive CTL clone HG-38

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MHC Class II

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Autre désignation: MHC-II

Sujet: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Poids moléculaire: 60 kDa

ID gène: 3117, 3118, 3119

UniProt: P01908, P01909, P01920