MHC Class II anticorps (CF®740)

N° du produit ABIN7656453

Cet anticorps anti-MHC Class II est un anticorps Souris Monoclonal détectant MHC Class II dans . Adapté pour Humain et Singe.

Aperçu rapide pour MHC Class II anticorps (CF®740) (ABIN7656453)

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II est conjugé à/à la CF®740

Application: Veuillez nous consulter SVP

Clone: IPO-10

Détail du produit anti-MHC Class II anticorps (CF®740)

Fonction: MHC II (HLA-DRA)(IPO-10), CF740 conjugate

Attributs du produit: MAb IPO-10 defines the antigen that appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50 % of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70 % of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

Isotype: IgG3, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Commentaires:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MHC Class II

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Autre désignation: MHC-II

Sujet:

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Gene Symbol: HLA-DRA

Poids moléculaire: 36 kDa

ID gène: 3122

UniProt: P01903