MHC Class II anticorps (CF®740)

N° du produit ABIN7656454

Cet anticorps Souris Monoclonal détecte spécifiquement MHC Class II dans FACS et IF. Il présente une réactivité envers Humain, Boeuf (Vache) et Cat.

Aperçu rapide pour MHC Class II anticorps (CF®740) (ABIN7656454)

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivité: Humain, Boeuf (Vache), Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II est conjugé à/à la CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 169-1B5-2

Détail du produit anti-MHC Class II anticorps (CF®740)

Fonction: MHC II (HLA-DRA)(169-1B5.2), CF740 conjugate

Attributs du produit: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human peripheral blood leukocytes

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MHC Class II

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Autre désignation: MHC-II

Sujet:

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Gene Symbol: HLA-DRA

Poids moléculaire: 36 kDa (? chain) and 27 kDa (? chain)

ID gène: 3122

UniProt: P01903