MVP anticorps (CF®740)

N° du produit ABIN7656492

Cet anticorps anti-MVP est un anticorps Souris Monoclonal détectant MVP dans WB et IF. Adapté pour Humain.

Aperçu rapide pour MVP anticorps (CF®740) (ABIN7656492)

Antigène: MVP (Major Vault Protein (MVP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MVP est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1014

Détail du produit anti-MVP anticorps (CF®740)

Fonction: Major Vault Protein (MVP)(1014), CF740 conjugate

Attributs du produit: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Proteins precipitated from human breast cancer MCF-7 cells

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: MCF-7/HeLa, breast tumors

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MVP

Antigène: MVP (Major Vault Protein (MVP))

Autre désignation: Major Vault Protein

Sujet:

Synonyms: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Gene Symbol: MVP

Poids moléculaire: 104-110 kDa

ID gène: 9961

UniProt: Q14764