Maltose Binding Protein anticorps (CF®594)

N° du produit ABIN7656516

Cet anticorps anti-Maltose Binding Protein est un anticorps Souris Monoclonal détectant Maltose Binding Protein dans WB. Adapté pour Différentes espèces.

Aperçu rapide pour Maltose Binding Protein anticorps (CF®594) (ABIN7656516)

Antigène: Maltose Binding Protein (MBP)

Reactivité: Différentes espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Maltose Binding Protein est conjugé à/à la CF®594

Application: Western Blotting (WB)

Clone: R29-6

Détail du produit anti-Maltose Binding Protein anticorps (CF®594)

Fonction: Maltose Binding Protein / MBP-probe(R29.6), CF594 conjugate

Attributs du produit: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: MOS maltose binding protein fusion protein.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.

Commentaires:

Positive Control: Saos-2 cells. MBP fusion proteins.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Maltose Binding Protein

Antigène: Maltose Binding Protein (MBP)

Autre désignation: Maltose Binding Protein

Sujet:

Synonyms: ECK4026, JW3994, MalE, malJ, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, Periplasmic maltose binding protein

Gene Symbol: Not Known

Poids moléculaire: 40 kDa