MILK FAT GLOBULIN anticorps (CF®740)

N° du produit ABIN7657115

L’anticorps Souris Monoclonal anti- a été validé pour IF, IHC, IHC (fp) et WB. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour MILK FAT GLOBULIN anticorps (CF®740) (ABIN7657115)

Antigène: MILK FAT GLOBULIN

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: CF®740

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Clone: MFG-06

Détail du produit

Fonction: Milk Fat Globulin(MFG-06), CF740 conjugate

Attributs du produit: Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human milk fat globule membrane preparation

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Normal breast or breast carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détail du antigène

Antigène: MILK FAT GLOBULIN

Autre désignation: Milk-Fat-Globulin

Sujet:

Synonyms: Breast epithelial antigen BA46, EDIL1, HMFG, Lactadherin, Lactadherin precursor, Medin, MFGE8, MFGM, Milk fat globule-EGF factor 8 (MFG-E8)

Gene Symbol: MFGE8

Tissue Expression: Epithelial cells|Mammary gland

Poids moléculaire: 45 kDa

ID gène: 4240

UniProt: Q08431