MUC2 anticorps (CF®740)

N° du produit ABIN7657369

Cet anticorps Souris Monoclonal détecte spécifiquement MUC2 dans IHC, WB, IF, ELISA et IHC (fp). Il présente une réactivité envers Humain.

Aperçu rapide pour MUC2 anticorps (CF®740) (ABIN7657369)

Antigène: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MUC2 est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CCP58

Détail du produit anti-MUC2 anticorps (CF®740)

Fonction: MUC2(CCP58), CF740 conjugate

Attributs du produit: Recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: LS174T cells or small intestine.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MUC2

Antigène: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Autre désignation: MUC2

Sujet:

Synonyms: Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC

Gene Symbol: MUC2

Tissue Expression: Goblet cells|Intestine

Poids moléculaire: 520 kDa

ID gène: 4583

UniProt: Q02817