MYOD1 anticorps (CF®740)

N° du produit ABIN7657620

Cet anticorps anti-MYOD1 est un anticorps Souris Monoclonal détectant MYOD1 dans . Adapté pour Humain, Souris, Rat et Poulet.

Aperçu rapide pour MYOD1 anticorps (CF®740) (ABIN7657620)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est conjugé à/à la CF®740

Application: Veuillez nous consulter SVP

Clone: 5-8A

Détail du produit anti-MYOD1 anticorps (CF®740)

Fonction: MyoD1(5.8A), CF740 conjugate

Attributs du produit: Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant mouse MyoD1 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry. Only nuclear staining should be considered as evidence of skeletal muscle differentiation. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Rhabdomyosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD1

Sujet:

Synonyms: bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Gene Symbol: MYOD1

Tissue Expression: Muscle

Poids moléculaire: 45 kDa

ID gène: 4654

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development