NEFH anticorps (Phosphospecific) (CF®740)

N° du produit ABIN7657904

L’anticorps Souris Monoclonal anti-NEFH a été validé pour WB, IHC, FACS et IHC (fp). Il convient pour détecter NEFH dans des échantillons de Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille et Cat.

Aperçu rapide pour NEFH anticorps (Phosphospecific) (CF®740) (ABIN7657904)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NE14

Détail du produit anti-NEFH anticorps (CF®740)

Épitope: Phosphospecific

Fonction: Neurofilament, phospho (NF-H) (Neuronal Marker)(NE14), CF740 conjugate

Attributs du produit: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Immunogène: Crude neurofilament preparation from porcine spinal cord

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.25-0.5 μg/mL for 30 minutes at RT. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HEK293 cells, Brain, Neuroblastoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: Neurofilament H

Sujet:

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200

Gene Symbol: NEFH

Tissue Expression: Neuroendocrine cells|Neurons

Poids moléculaire: 200 kDa

ID gène: 4744

UniProt: P12036