NKX6-1 anticorps (Biotin)

N° du produit ABIN7658055

Cet anticorps anti-NKX6-1 est un anticorps Souris Monoclonal détectant NKX6-1 dans IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour NKX6-1 anticorps (Biotin) (ABIN7658055)

Antigène: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NKX6-1 est conjugé à/à la Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NKX61-2561

Détail du produit anti-NKX6-1 anticorps (Biotin)

Fonction: NKX6.1 (Marker for Pancreatic and Duodenal Neuroendocrine Tumors) (NKX61/2561), Biotin conjugate

Attributs du produit: Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature B cells, which produce Insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic B cell specific genes, including Insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in B cells.Nkx6.1 is highly expressed in pancreatic and duodenal well-differentiated neuroendocrine tumors (WDNETS) and in metastatic WDNETS, is a highly specific marker of tumors of pancreatic origin. It has thus been suggested that Nkx6.1 is a useful inclusion into IHC panels for identifying primary sites of WDNETS.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human full-length recombinant NKX6.1 protein

Isotype: IgG2c, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Human fetal small intestine lysate or pancreas tissue.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur NKX6-1

Antigène: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Autre désignation: NKX6.1

Sujet:

Synonyms: Homeobox protein NK-6 homolog A, Homeobox protein Nkx-6.1, NK homeo box, family 6, member A, NK homeobox (Drosophila), family 6, A, NK homeobox, family 6, A, NK6 homeobox 1, NK6 transcription factor homolog A, NK6 transcription factor related, locus 1, NK6 transcription factor related, locus 1 (Drosophila), NKX6-1, NKX6.1, NKX61, NKX61_HUMAN, NKX6A

Gene Symbol: NKX6-1

Tissue Expression: Pancreatic beta cells

Poids moléculaire: 40-50 kDa

ID gène: 4825

UniProt: P78426

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion