Occludin anticorps (AA 282-415) (CF®740)

N° du produit ABIN7658203

Cet anticorps Souris Monoclonal détecte spécifiquement Occludin dans WB, IHC et IHC (fp). Il présente une réactivité envers Humain.

Aperçu rapide pour Occludin anticorps (AA 282-415) (CF®740) (ABIN7658203)

Antigène: Occludin (OCLN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Occludin est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: OCLN-2181

Détail du produit anti-Occludin anticorps (CF®740)

Épitope: AA 282-415

Fonction: Occludin (Marker of Early Blood Brain Barrier Damage)(OCLN/2181), CF740 conjugate

Attributs du produit: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment of human Occludin protein (around aa 282-415)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: HepG2 cells. Kidney.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: Occludin

Sujet:

Synonyms: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin

Gene Symbol: OCLN

Tissue Expression: Microvascular endothelial cells

Poids moléculaire: 60-82 kDa

ID gène: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C