pan Keratin anticorps (CF®740)

N° du produit ABIN7658422

Cet anticorps Souris Monoclonal détecte spécifiquement pan Keratin dans IF, FACS, WB, IHC et IHC (fp). Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache), Porc, Grenouille, Cobaye, Chévre et Marmoset.

Aperçu rapide pour pan Keratin anticorps (CF®740) (ABIN7658422)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Porc, Grenouille, Cobaye, Chévre, Marmoset

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est conjugé à/à la CF®740

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: C11

Détail du produit anti-pan Keratin anticorps (CF®740)

Fonction: Cytokeratin, multi(C11), CF740 conjugate

Attributs du produit: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Keratin-enriched preparation from cultured human A431

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1.0 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: A431 cells, Skin, Colon carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Keratin, multi

Sujet:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT4

Tissue Expression: Epidermal cells|Epithelial cells

Poids moléculaire: Multiple

ID gène: 3851