pan Keratin anticorps (High Molecular Weight) (CF®740)

N° du produit ABIN7658423

L’anticorps Souris Monoclonal anti-pan Keratin a été validé pour IF, IHC, WB et IHC (fp). Il convient pour détecter pan Keratin dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour pan Keratin anticorps (High Molecular Weight) (CF®740) (ABIN7658423)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est conjugé à/à la CF®740

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 34BE12

Détail du produit anti-pan Keratin anticorps (CF®740)

Épitope: High Molecular Weight

Fonction: Cytokeratin, HMW(34BE12), CF740 conjugate

Attributs du produit: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Solubilized keratin extract from human stratum corneum

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: PC12 cells, Skin, Prostate carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Keratin

Sujet:

Synonyms: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Gene Symbol: KRT76

Tissue Expression: Epidermal cells|Epithelial cells

Poids moléculaire: 50-67 kDa

ID gène: 51350

UniProt: Q01546