Recombinant pan Keratin anticorps (acidic) (CF®740)

N° du produit ABIN7658431

Cet anticorps Lapin Monoclonal détecte spécifiquement pan Keratin dans IHC et IHC (fp). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant pan Keratin anticorps (acidic) (CF®740) (ABIN7658431)

Antigène: pan Keratin (panKRT)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KRTL-1577R

Détail du produit anti-pan Keratin anticorps (CF®740)

Épitope: acidic

Fonction: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (KRTL/1577R), CF740 conjugate

Attributs du produit: This MAb recognizes the 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human KRT77 protein fragment

Isotype: IgG, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin) 1-2 μg/mL. Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: Skin, Squamous cell carcinoma (SCC)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Keratin

Sujet:

Synonyms: K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B

Gene Symbol: KRT77

Tissue Expression: Epidermal cells|Epithelial cells

Poids moléculaire: 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)

ID gène: 374454

UniProt: Q7Z794