Plakophilin 1 anticorps (N-Term) (CF®740)

N° du produit ABIN7658623

Cet anticorps Souris Monoclonal détecte spécifiquement Plakophilin 1 dans WB. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Plakophilin 1 anticorps (N-Term) (CF®740) (ABIN7658623)

Antigène: Plakophilin 1 (PKP1)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Plakophilin 1 est conjugé à/à la CF®740

Application: Western Blotting (WB)

Clone: 10B2

Détail du produit anti-Plakophilin 1 anticorps (CF®740)

Épitope: N-Term

Fonction: Plakophilin-1 (PKP1) (10B2), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: N-terminus of human Plakophilin-1 protein

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HaCat Cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Plakophilin 1

Antigène: Plakophilin 1 (PKP1)

Autre désignation: Plakophilin-1

Sujet:

Synonyms: B6P, B, 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

Gene Symbol: PKP1

Tissue Expression: Cardiac

Poids moléculaire: 75 kDa

ID gène: 5317

UniProt: Q13835