Recombinant TYMS anticorps (AA 60-174) (CF®647)

N° du produit ABIN7660725

Cet anticorps anti-TYMS est un anticorps Souris Monoclonal détectant TYMS dans IF et FACS. Adapté pour Humain.

Aperçu rapide pour Recombinant TYMS anticorps (AA 60-174) (CF®647) (ABIN7660725)

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYMS est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RTYMS-1884

Détail du produit anti-TYMS anticorps (CF®647)

Épitope: AA 60-174

Fonction: Thymidylate Synthase (5-FU Resistance Marker)(rTYMS/1884), CF647 conjugate

Attributs du produit: Recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2. 1. 1. 45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5, 10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human thymidylate synthase protein fragment (around aa 60-174)

Isotype: IgG1, kappa

Information d'application

Indications d'application: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Immunofluorescence: 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Commentaires:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur TYMS

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Autre désignation: Thymidylate Synthase

Classe de substances: Viral Protein

Sujet:

Synonyms: dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Gene Symbol: TYMS

Poids moléculaire: 36 kDa

ID gène: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases