TIA1 anticorps (AA 279-380) (CF®740)

N° du produit ABIN7660832

L’anticorps Souris Monoclonal anti-TIA1 a été validé pour WB et IF. Il convient pour détecter TIA1 dans des échantillons de Humain.

Aperçu rapide pour TIA1 anticorps (AA 279-380) (CF®740) (ABIN7660832)

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIA1 est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TIA1-1313

Détail du produit anti-TIA1 anticorps (CF®740)

Épitope: AA 279-380

Fonction: TIA1 (T-Cell-Restricted Intracellular Antigen-1) (TIA1/1313), CF740 conjugate

Attributs du produit: TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)

Isotype: IgG2b

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HepG2, PC3 and HeLa cells. Tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur TIA1

Antigène: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Autre désignation: TIA1

Sujet:

Synonyms: Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, T-cell-restricted intracellular antigen-1 (TIA1), TIA-1, TIA1, TIA1 cytotoxic granule associated RNA binding protein, TIAL1, TIAR, WDM

Gene Symbol: TIA1

Poids moléculaire: 40 kDa

ID gène: 7072

UniProt: P31483