Transferrin anticorps

N° du produit ABIN7661012

L’anticorps Souris Monoclonal anti-Transferrin a été validé pour IHC et IHC (fp). Il convient pour détecter Transferrin dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Transferrin anticorps (ABIN7661012)

Antigène: Transferrin (TF)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: A78-G-A7

Détail du produit anti-Transferrin anticorps

Fonction: Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)(A78-G/A7)

Attributs du produit: This antibody recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA-free and azide-free (1 mg/mL).

Immunogène: Neuraminidase-treated human red blood cells

Isotype: IgM, kappa

Information d'application

Indications d'application: Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: KG1 cells. Human colorectal carcinoma tissues.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Transferrin

Antigène: Transferrin (TF)

Autre désignation: CD176

Sujet:

Synonyms: T-F Antigen, TF Antigen, Asialoglycophorin, pan-carcinoma marker, CD176

Gene Symbol: Not Applicable

Poids moléculaire: Multiple

Pathways: Transition Metal Ion Homeostasis