Transgelin anticorps (CF®740)

N° du produit ABIN7661060

L’anticorps Souris Monoclonal anti-Transgelin a été validé pour IHC et IHC (fp). Il convient pour détecter Transgelin dans des échantillons de Humain, Souris, Lapin, Boeuf (Vache) et Porc.

Aperçu rapide pour Transgelin anticorps (CF®740) (ABIN7661060)

Antigène: Transgelin (TAGLN)

Reactivité: Humain, Souris, Lapin, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transgelin est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: TAGLN-247

Détail du produit anti-Transgelin anticorps (CF®740)

Fonction: Transgelin / SM22-alpha(TAGLN/247), CF740 conjugate

Attributs du produit: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human transgelin (TAGLN) protein.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Commentaires:

Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Transgelin

Antigène: Transgelin (TAGLN)

Autre désignation: SM22-Alpha

Sujet:

Synonyms: 2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10

Gene Symbol: TAGLN

Tissue Expression: Smooth muscle

Poids moléculaire: 22 kDa

ID gène: 6876

UniProt: Q01995