Villin 1 anticorps (AA 179-311) (CF®740)

N° du produit ABIN7661894

L’anticorps Souris Monoclonal anti-Villin 1 a été validé pour IHC, WB, IF, FACS et IHC (fp). Il convient pour détecter Villin 1 dans des échantillons de Humain.

Aperçu rapide pour Villin 1 anticorps (AA 179-311) (CF®740) (ABIN7661894)

Antigène: Villin 1 (VIL1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Villin 1 est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: VIL1-1325

Détail du produit anti-Villin 1 anticorps (CF®740)

Épitope: AA 179-311

Fonction: Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/1325), CF740 conjugate

Attributs du produit: This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human Villin fragment of 133 amino acid residues (aa179-311)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunohistology (formalin): 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: Villin

Sujet:

Synonyms: VIL1, Villin-1, Villin1

Gene Symbol: VIL1

Tissue Expression: Epithelial cells|Intestine

Poids moléculaire: 93 kDa

ID gène: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization