VWF anticorps (AA 845-949) (CF®647)

N° du produit ABIN7661982

Cet anticorps anti-VWF est un anticorps Souris Monoclonal détectant VWF dans IHC et IHC (fp). Adapté pour Humain.

Aperçu rapide pour VWF anticorps (AA 845-949) (CF®647) (ABIN7661982)

Antigène: VWF (Von Willebrand Factor (VWF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VWF est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: IIIE2-34

Détail du produit anti-VWF anticorps (CF®647)

Épitope: AA 845-949

Fonction: von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)(IIIE2.34), CF647 conjugate

Attributs du produit: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Immunogène: Recombinant human vWF fragment spanning aa 845-949

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Western blot: 0.5-1.0 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HUVEC cells. Tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur VWF

Antigène: VWF (Von Willebrand Factor (VWF))

Autre désignation: Von Willebrand Factor

Sujet:

Synonyms: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Gene Symbol: VWF

Tissue Expression: Endothelial cells

Poids moléculaire: 250 kDa

ID gène: 7450

UniProt: P04275