WT1 anticorps (AA 1-181) (CF®740)

N° du produit ABIN7662042

Cet anticorps anti-WT1 est un anticorps Souris Monoclonal détectant WT1 dans IHC et IHC (fp). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour WT1 anticorps (AA 1-181) (CF®740) (ABIN7662042)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp WT1 est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 6F-H2

Détail du produit anti-WT1 anticorps (CF®740)

Épitope: AA 1-181

Fonction: WT1 (Wilm's Tumor 1)(6F-H2), CF740 conjugate

Attributs du produit: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant protein corresponding to residues 1-181 of human WT1.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: Wilms Tumor 1

Sujet:

Synonyms: WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1

Gene Symbol: WT1

Poids moléculaire: 47-55 kDa

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation