EZH2 anticorps

N° du produit ABIN7673034

L’anticorps Souris Monoclonal anti-EZH2 a été validé pour WB et ELISA. Il convient pour détecter EZH2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EZH2 anticorps (ABIN7673034)

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EZH2 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 5G4

Détail du produit anti-EZH2 anticorps

Fonction: EZH2 antibody

Purification: protein A+G purified

Pureté: ≥95 % as determined by SDS-PAGE

Immunogène: enhancer of zeste homolog 2(Drosophila)

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:1000-1:4000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Stockage commentaire: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Date de péremption: 12 months

Détails sur EZH2

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Autre désignation: EZH2

Sujet:

Synonyms: Histone-lysine N-methyltransferase EZH2|ENX-1|Enhancer of zeste homolog 2|Lysine N-methyltransferase 6|EZH2|KMT6

Background: Polycomb group(PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9'(H3K9me) and 'Lys-27'(H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di-and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer, involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Poids moléculaire: 90-102 kDa

ID gène: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation