SARS-CoV-2 Spike anticorps (N-Term) (HRP)

N° du produit ABIN7795006

Cet anticorps anti-SARS-CoV-2 Spike est un anticorps Souris Monoclonal détectant SARS-CoV-2 Spike dans IHC. Adapté pour SARS Coronavirus-2 (SARS-CoV-2).

Aperçu rapide pour SARS-CoV-2 Spike anticorps (N-Term) (HRP) (ABIN7795006)

Antigène: SARS-CoV-2 Spike

Reactivité: SARS Coronavirus-2 (SARS-CoV-2)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SARS-CoV-2 Spike est conjugé à/à la HRP

Application: Immunohistochemistry (IHC)

Clone: 2146

Détail du produit anti-SARS-CoV-2 Spike anticorps (HRP)

Épitope: N-Term

Fonction: Anti-SARS-CoV-2 Spike NTD (Clone 2146) HRP

Specificité: Anti-SARS-CoV-2 Spike NTD-HRP, clone 2146, specifically targets an epitope on the SARS-CoV-2 spike protein N-terminal domain. Antigen Distribution The spike NTD is expressed on the surface of SARS-CoV-2. Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6 % identity with SARS-CoV, encodes four essential structural proteins the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). In both SARS-CoV and SARS-CoV-2, the CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells3-5. The NTD of SARS-CoV-2 does not bind to ACE26, and the function of NTD in SARS-CoV-2 infection is not well understood. In other CoVs, the NTD may promote attachment by binding to sugar moieties7 and might play a role in the conformational change of S2 required for membrane fusion8. While most neutralizing antibodies target the RBD domain and block receptor binding, potent neutralizing antibodies targeting NTD were isolated from convalescent COVID19 patients9, identifying the NTD as an attractive candidate for vaccines and therapeutics. In addition, the NTD is a promising antigen for diagnostic tests, as there is only 53.5 % homology between the NTD of SARS-CoV-2 and SARS-CoV10.

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/mL

Buffer: This recombinant HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1 % BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze.

Détails sur SARS-CoV-2 Spike

Antigène: SARS-CoV-2 Spike

Classe de substances: Viral Protein

Sujet: COV2-2146, SARS-CoV2 Spike NTD, COVID-19, 2019-nCoV, Severe acute respiratory syndrome coronavirus 2, SARS-CoV2,