L’anticorps anti-ARID1A Monoclonal produit chez le Lapin a été validé pour WB, IHC, ICC, IP et FACS. Il convient pour la détection de ARID1A dans des échantillons provenant de Humain et Souris.
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ARID1A
Reactivité: Humain, Souris
IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,IP,20 μL/mg lysate ,WB,11000 (3-8 % Tris-acetate gel recommended)
Restrictions
For Research Use only
Format
Liquid
Concentration
50 μg/mL
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1A/BAF250 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1A/BAF250 has been shown to play a role in the tumor suppressor activity of the SWI/SNF complexes. ARID1A/BAF250 is required for differentiation-associated cell cycle arrest.