Atp1b2/Na+K+Atpase (AA 201-290) anticorps

N° du produit ABIN738426

Détail du produit

Antigène: Atp1b2/Na+K+Atpase

Épitope: AA 201-290

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC)

Réactivité croisée: Humain, Souris, Rat

Homologie: Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human ATP1b2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Independent Validation (IF)

Validation #100012 (Immunofluorescence)

by: Memorial Sloan-Kettering Cancer Center, Parada lab

No.: #100012

Date: 11.05.2016

Antigène: Atp1b2/Na+K+Atpase (ATP1B2)

Application validée: Immunofluorescence

Contrôle positif: Wild-type mouse adult brain cryo-sections

Contrôle négative: Rabbit IgG

Conclusion: The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.

Validation Images

IF validation of ATP1B2 antibody ABIN738426 on mouse brain sections. A. ABIN738426 diluted 1:400 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:200. D. Dilution factor 1:400.

Protocole

Anticorps primaire: ABIN738426

Anticorps secondaire: Donkey-anti-Rabbit-Cy3

Full Protocol:

• Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
• Immunostaining for Cryo Sections:
• • Dry slides at 50-55°C for 15-30min (or RT for 30min).
  • Wash with PBS 5min X 3 (shake gently).
  • Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
  • Incubate with ABIN738426 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
  • Wash with PBS 5min X 3.
  • Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
  • Wash with PBS 5min X 3.
  • Add three drops of water-based Immu-mount.
  • Cover with 24 X 60 cover glass.
• Imaging:
• • Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Notes: ABIN738426 worked within 1:50–1:400, worked well at 1:400.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Atp1b2/Na+K+Atpase

Sujet:

Synonyms: AMOG, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, Sodium/potassium-dependent ATPase subunit beta-2, ATP1B2

Background: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.

ID gène: 482

UniProt: P14415