Feimin anticorps

N° du produit ABIN7822917

N° du produit (Fournisseur): 200-401-nh2

L’anticorps anti-Feimin Polyclonal Lapin est utilisé pour la détection de Feimin dans des échantillons de Humain et Souris. Il a été validé pour ELISA, IP et WB.

Aperçu rapide pour Feimin anticorps (ABIN7822917)

Antigène: Feimin

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 200-401-nh2

Fournisseur: Rockland

Fonction: Anti-Feimin (RABBIT) Antibody

Réactivité croisée (Details): A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.

Purification: Anti-Feimin Antibody is purified from monospecific antiserum by Protein A affinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant mouse Feimin protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Feimin (RABBIT) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T and E. coli). A band approximately 20.1kDa in size corresponding to C5orf24 is detected. Cell lines expressing feimin can be expected using appropriate tissues and lysates. Specific conditions for reactivity should be optimized by the end user.

Commentaires:

ELISA Dilution: 1:44,500-1:64,500
Western Blot Dilution: 40 ng/mL
Immunoprecipitation Dilution: 40 ng/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.18 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: Feimin

Sujet: Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin

ID gène: 134553

NCBI Accession: NP_001129058.1

UniProt: Q80X32