Recombinant L1CAM anticorps (AA 550-900)
Aperçu rapide pour Recombinant L1CAM anticorps (AA 550-900) (ABIN7826108)
Antigène
Voir toutes L1CAM AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 550-900
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Fonction
- CD171 antibody (171R)
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Immunogène
- Recombinant fragment (around aa550-900) of human L1CAM protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Kidney: A strong membranous L1CAM staining should be seen in a subset of collecting ducts while the vast majority of tubuli remains L1CAM negative.
Product Description/Background: CD171 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Kidney: L1CAM staining must be absent in the vast majority of tubuli (L1CAM staining is limited to collecting ducts).
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Date de péremption
- 24 months
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- L1CAM (L1 Cell Adhesion Molecule (L1CAM))
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Autre désignation
- CD171
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Sujet
- Gene Alias: CAML1, Neural cell adhesion molecule L1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve- growth factor-inducible large external glycoprotein, NILE, S10, SPG1, Neural cell adhesion molecule L1
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UniProt
- P32004
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Pathways
- Synaptic Membrane
Antigène
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