Recombinant Claudin 3 anticorps (Intracellular)
Aperçu rapide pour Recombinant Claudin 3 anticorps (Intracellular) (ABIN7826112)
Antigène
Voir toutes Claudin 3 (CLDN3) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- Intracellular
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Fonction
- Claudin 3 antibody (HMV309)
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Immunogène
- Synthetic peptide encompassing a sequence within the Intracellular domain of human Claudin 3. The exact sequence is proprietary.
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Liver: A strong membranous CLDN3 staining should be seen in bile ducts. In addition, a weak to moderate membranous CLDN3 staining should be seen in hepatocytes (often seen predominantly at the bile secreting pole).
Product Description/Background: Claudin 3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Stomach: Most epithelial cells should be CLDN3 negative, while a strong membranous CLDN3 staining of a small subset of epithelial cells in the neck and in glandular pits must be seen.
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Date de péremption
- 24 months
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- Claudin 3 (CLDN3)
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Autre désignation
- Claudin 3
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Sujet
- Gene Alias: claudin 3,C7orf1,CPE-R2,CPETR2,HRVP1,RVP1
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UniProt
- O15551
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Pathways
- Hepatitis C
Antigène
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