Recombinant RNF40 anticorps

N° du produit ABIN7828533

Cet anticorps anti-RNF40 Monoclonal Lapin (Clone A775) (ABIN7828533) détecte spécifiquement RNF40 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant RNF40 anticorps (ABIN7828533)

Antigène: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp RNF40 est non-conjugé

Application: Western Blotting (WB)

Clone: A775

Détail du produit anti-RNF40 anticorps

Fonction: Recombinant RNF40 Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Information d'application

Indications d'application: WB 1:1000

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Date de péremption: 12 months

Détails sur RNF40

Antigène: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Autre désignation: RNF40

Sujet: RBP,RNF,KIAA,RNF40,BRE1B,RBP95,STARING,KIAA0661,In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1. Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin. Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified. These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways. Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Poids moléculaire:

Calculated MW: 114 kDa

Observed MW: 140 kDa The actual band is not consistent with the expectation.

UniProt: O75150