mNeonGreen anticorps

N° du produit ABIN7833426

Cet anticorps Lapin Polyclonal détecte spécifiquement mNeonGreen dans IF et WB. Il présente une réactivité avec des échantillons de Arabidopsis thaliana.

Aperçu rapide pour mNeonGreen anticorps (ABIN7833426)

Antigène: mNeonGreen

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Western Blotting (WB)

Détail du produit

Fonction: Anti-mNeonGreen | Fluorescent Protein

Réactivité croisée (Details): mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known.

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated peptide derived from synthetic peptide

Information d'application

Indications d'application: 1 μg/1 mL (IF), 1 : 1000 - 1: 5000 (WB)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL, of sterile water.

Buffer: Immunogen affinity purified serum, in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: mNeonGreen

Sujet:

Background: mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.

Additional information: The peptide used to elicit this antibody is conserved in the following expression vectors: dCas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM020]Cas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM006]mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]mNeonGreen, partial [Binary vector pRATIO2131]The peptide used to elicit this antibody is not conserved in GFP protein sequence. Antibody is also recognzing mCheery sequence.

Poids moléculaire: Depends upon used fusion partner

UniProt: A0A1S4NYF2