ICAM2 anticorps (PE)

N° du produit ABIN7835571

Cet anticorps Rat Monoclonal détecte spécifiquement ICAM2 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour ICAM2 anticorps (PE) (ABIN7835571)

Antigène: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM2 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3C4

Détail du produit anti-ICAM2 anticorps (PE)

Fonction: Rat monoclonal antibody PE labled to CD102

Specificité: Recognizes mouse CD102

Purification: The antibody was purified by affinity chromatography.

Immunogène: COS cells transfected with mouse ICAM-2 cDNA

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur ICAM2

Antigène: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Autre désignation: ICAM2

Sujet: Intercellular adhesion molecule 2, ICAM-2, CD102

ID gène: 15896