LAMP2 anticorps (Alexa Fluor 647)

N° du produit ABIN7835614

L’anticorps anti-LAMP2 Monoclonal Souris est utilisé pour la détection de LAMP2 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour LAMP2 anticorps (Alexa Fluor 647) (ABIN7835614)

Antigène: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP2 est conjugé à/à la Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H4B4

Détail du produit anti-LAMP2 anticorps (Alexa Fluor 647)

Fonction: Mouse monoclonal antibody AF647 labled to CD107b

Specificité: Recognizes human CD107b

Purification: The antibody was purified by affinity chromatography.

Immunogène: Adult human adherent spleen cells

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur LAMP2

Antigène: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Autre désignation: LAMP2

Sujet: Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, CD107b

ID gène: 3920

UniProt: P13473

Pathways: Autophagy