CD109 anticorps

N° du produit ABIN7835626

L’anticorps anti-CD109 Monoclonal Souris est utilisé pour la détection de CD109 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD109 anticorps (ABIN7835626)

Antigène: CD109

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD109 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: W7C5

Détail du produit anti-CD109 anticorps

Fonction: Mouse monoclonal antibody to CD109

Specificité: Recognizes human CD109

Purification: The antibody was purified by affinity chromatography.

Immunogène: WERI-RB-1 retinoblastoma cell line

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD109

Antigène: CD109

Autre désignation: CD109

Sujet: CPAMD7, CD109 antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150, CD109

ID gène: 135228

UniProt: Q6YHK3

Pathways: Activated T Cell Proliferation