CD151 anticorps

N° du produit ABIN7835783

Cet anticorps anti-CD151 Monoclonal Souris (Clone 50-6) (ABIN7835783) détecte spécifiquement CD151 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD151 anticorps (ABIN7835783)

Antigène: CD151

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD151 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 50-6

Détail du produit anti-CD151 anticorps

Fonction: Mouse monoclonal antibody to CD151

Specificité: Recognizes human CD151

Purification: The antibody was purified by affinity chromatography.

Immunogène: Human epidermoid carcinoma cell line HEp-3

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD151

Antigène: CD151

Autre désignation: CD151

Sujet: TSPAN24, CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, PETA-3, Tetraspanin-24, Tspan-24, CD151

ID gène: 977

UniProt: P48509