Fc Receptor, IgG, Low Affinity IV (FCGR4) anticorps

N° du produit ABIN7835824

L’anticorps anti-Fc Receptor, IgG, Low Affinity IV Monoclonal Hamster arménien est utilisé pour la détection de Fc Receptor, IgG, Low Affinity IV dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour Fc Receptor, IgG, Low Affinity IV (FCGR4) anticorps (ABIN7835824)

Antigène: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 9E9

Détail du produit

Fonction: Armenian Hamster monoclonal antibody to CD16.2

Specificité: Recognizes mouse CD16.2

Purification: The antibody was purified by affinity chromatography.

Immunogène: FCgammaR4 -EC domain fusion with IgG1 Fc

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détail du antigène

Antigène: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Autre désignation: FCGR4

Sujet: Low affinity immunoglobulin gamma Fc region receptor IV, CD16-2, FcgammaRIV

ID gène: 246256