CD163 anticorps (APC)

N° du produit ABIN7835846

Cet anticorps anti-CD163 Monoclonal Souris conjugué à APC (Clone GHI-61) (ABIN7835846) détecte spécifiquement CD163 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD163 anticorps (APC) (ABIN7835846)

Antigène: CD163

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GHI-61

Détail du produit anti-CD163 anticorps (APC)

Fonction: Mouse monoclonal antibody APC labled to CD163

Specificité: Recognizes human CD163

Purification: The antibody was purified by affinity chromatography.

Immunogène: Human monocytes

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163

ID gène: 9332

UniProt: Q86VB7