CXCR3 anticorps (N-Term)

N° du produit ABIN7835875

L’anticorps anti-CXCR3 Monoclonal Hamster arménien est utilisé pour la détection de CXCR3 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour CXCR3 anticorps (N-Term) (ABIN7835875)

Antigène: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CXCR3 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CXCR3-173

Détail du produit anti-CXCR3 anticorps

Épitope: N-Term

Fonction: Armenian Hamster monoclonal antibody to CD183

Specificité: Recognizes mouse CD183

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse N-terminus of CXCR3

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-Armenian Hamster IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CXCR3

Antigène: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Autre désignation: CXCR3

Sujet: GPR9, C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, CKR-L2, G protein-coupled receptor 9, Interferon-inducible protein 10 receptor, IP-10 receptor, CD183

ID gène: 12766